Appendix 2.  Geological and Metallurgical Reference Materials
Appendix 2.3 Soils and sediments
Matrix Analytes Reagents Cavity/Vessel MW conditions Detection Ref.
BCR 

Calcareous loam 

141

Cd, Cr, Cu, Fe, Mn, Pb, Zn HCl, HNO3 Multimode/ 

LP closed

1 min at 180 W, 4 min at 480 W, 

60 min at 600 W

F-AAS 

ETV-AAS

F35
Cd, Cu, Pb 1. HNO3, H2O, H2O2 

2. Aqua regia, HF 

(optional)

Multimode/ 

open

1. 15 min at 70 W, 

15 min at 150 W, 

15 min at 180 W, 

1 min at 250 W, 

1 min at 600 W 

2. 15 min at 70 W, 

1 min at 600 W

ETV-AAS F36
BCR 

Soil - light sandy 

142

Cd, Cr, Cu, Fe, Mn, Pb, Zn HCl, HNO3 Multimode/ 

LP closed

1 min at 180 W, 

4 min at 480 W, 

60 min at 600 W

F-AAS 

ETV-AAS

F35
BCR 

Estuarine 

sediment 

277

As, Cd, Cr, Cu, Hg, Ni, Pb, Zn A. 

1. HCl, HNO3 

2. HCl, HNO3 

3. H2O2 

4. H2

B. 

HCl, HNO3, HF

Single mode/ 

open 
  

Multimode/ 

LP closed

1. 5 min at 40 W, 10 min at 50 W 

2. 10 min at 54 W 

3. 5 min at 40 W 

4. 5 min at 50 W 

10 min at 120 W, 

20 min at 240 W, 

20 min at 300 W

ICP-OES 

ICP-MS 

CV-AAS 

HPLC-ICP-MS

F37
CANMET 

Regosolic C 

horizon soil SO-1

As 1. HNO3 

2. HNO3, HClO4, H2SO4

Multimode/ 

LP closed

1. 2.5 min at 639 W, cool, vent 

2. 2.5 min at 639 W, 

2 min 0 W (x3)

F-AAS F38
Pb HNO3, HF Multimode/ 

LP closed

7 min at 400 W DP-ASV F39
CANMET 

Podzolic B 

horizon soil 

SO-2

As 1. HNO3 

2. HNO3, HClO4, H2SO4

Multimode/ 

LP closed

1. 2.5 min at 639 W, cool, vent 

2. 2.5 min at 639 W, 

2 min 0 W (x3)

F-AAS F38
Pb HNO3, HF Multimode/ 

LP closed

7 min at 400 W DP-ASV F39
CANMET 

Calcareous C 

horizon soil 

SO-3

As 1. HNO3 

2. HNO3, HClO4, H2SO4

Multimode/ 

LP closed

1. 2.5 min at 639 W, cool, vent 

2. 2.5 min at 639 W, 

2 min 0 W (x3)

F-AAS F38
CANMET 

Calcareous C 

horizon soil 

SO-3 

(contd.)

Pb HNO3, HF Multimode/ 

LP closed

7 min at 400 W DP-ASV F39
CANMET 

Black Chernozemic A horizon soil 

SO-4

As 1. HNO3 

2. HNO3, HClO4, H2SO4

Multimode/ 

LP closed

1. 2.5 min at 639 W, cool, vent 

2. 2.5 min at 639 W, 

2 min 0 W (x3)

F-AAS F38
Pb HNO3, HF Multimode/ 

LP closed

7 min at 400 W DP-ASV F39
IAEA 

Lake 

sediment SL-1

Al, Ba, Ca, Cr, Cu, Fe, K, Li, Mg, Mn, Na, P, Pb, Si, Sr, Ti, V, Zn Aqua regia, HF Multimode/ 

open

3 min at 625 W ICP-OES F21
Cd, Cr, Cu, Fe, Mn, Pb, Zn HCl, HNO3 Multimode/ 

LP closed

1 min at 180 W, 4 min at 480 W, 

60 min at 600 W

F-AAS 

ETV-AAS

F35
IAEA 

Soil 

5

Hg HCl, HNO3, H2O Multimode/ 

LP closed

1 min at 180 W, 4 min at 480 W, 

10 min at 600 W

CV-AAS 

NAA

F40
IAEA 

Soil 

7

Cd, Cr, Cu, Fe, Mn, Pb, Zn HCl, HNO3 Multimode/ 

LP closed

1 min at 180 W, 4 min at 480 W, 

60 min at 600 W

F-AAS 

ETV-AAS

F35
IGGE 

Stream sediment 

GSD-2

Al, Be, Cr, K, La, Li, Nb, Si, Sn, Ti, Y, Zr 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
IGGE 

Stream sediment 

GSD-3

Al, Cr, K, La, Li, Si, Ti, V, Zr 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
IGGE 

Stream sediment 

GSD-8

Al, K, Li, Nb, Si, Sn, Ti, Y, Zr 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
NIES 

Pond sediment 

2

Hg HNO3 Multimode/ 

HP closed

70 sec at 600 W CV-AAS F41
NIST 

River sediment 

1645

Ca, Cr, Fe, Mn, Pb, Zn Exchangeable: 

MgCl2 

Carbonate Bound: 

Sodium Acetate 

Fe-Mn Oxide: 

Hydroxylamine 

hydrochloride 

Organic: 

H2O2, HNO3 

Residual: 

HNO3, HCl, HF

Multimode/ 

open 

Multimode/ 

open 

Multimode/ 

open 

Multimode/ 

open 

Multimode/ 

open

4 min at ? W 

5 min at ? W 

4 min at ? W (x3) 

4 min at ? W 

5 min at ? W

F-AAS F42
Al, As, Ba, Ca, Cr, Cu, Fe, K, Mg, Mn, Na, P, Pb, Si, Sr, Ti, Zn Aqua regia, HF Multimode/ 

open

3 min at 625 W ICP-OES F21
Al, K, Li, Si 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
Th, U 1. HCl, HNO3, HF 

2. Aqua regia

Multimode/ 

HP closed

1. 4-8 min at 600 W, 

fume off acid (x3) 

2. If residue 3 min at 600 W

a spectrometry F7
Hg HNO3 Multimode/ 

LP closed

Heat to 75°C (800 W), 

2 min at 75°C

CV-FANES F43
As, Cd, Cu, Mg, Mn, Ni, Pb, Zn HNO3, H2O Multimode/ 

LP closed

Heat to 100 psi, 

55 min at 100 psi

- F44
Al, Ba, Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sc, Si, Ti, V, Zn, Zr 1. HF, Aqua regia 

2. H3BO3

Multimode/ 

LP closed

1. 8 min at 300 W, 

4 min at 600 W, 

7 min at 480 W 

2. 6 min at 600 W

ICP-OES F20
Al, As, Ba, Ca, Cd, Cr, Cu, Co, Fe, K, Mg, Mn, Na, Ni, Pb, Sb, Sc, Se, Si, Sn, Ti, Tl, V, Zn, Zr 1. HF, Aqua regia 

2. H3BO3

Multimode/ 

LP closed

1. 8 min at 300 W, 

11 min at 600 W 

2. 6 min at 300 W

ICP-OES 

F-AAS 

ETV-AAS

F6
NIST Estuarine sediment 

1646

Al, As, Ba, Ca, Cd, Cr, Cu, Co, Fe, K, Mg, Mn, Na, Ni, Pb, Sb, Sc, Se, Si, Sn, Ti, Tl, V, Zn, Zr 1. HF, Aqua regia 

2. H3BO3

Multimode/ 

LP closed

1. 8 min at 300 W, 

11 min at 600 W 

2. 6 min at 300 W

ICP-OES 

F-AAS 

ETV-AAS

F6
Ca, Cr, Fe, Mn, Pb, Zn Exchangeable: 

MgCl2 

Carbonate Bound: 

Sodium Acetate 

Fe-Mn Oxide: 

Hydroxylamine 

hydrochloride 

Organic: 

H2O2, HNO3 

Residual: 

HNO3, HCl, HF

Multimode/ 

open 

Multimode/ 

open 

Multimode/ 

open 

Multimode/ 

open 

Multimode/ 

open

4 min at ? W 

5 min at ? W 

4 min at ? W (x3) 

4 min at ? W 

5 min at ? W

F-AAS F42
Al, As, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Li, Mg, Mn, Na, Ni, Pb, Ti, V, Zn HNO3 Multimode/ 

LP closed

10 min at 400 W 

50 min at 330 W

ICP-OES F45
Al, Ba, Ca, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sc, Si, Ti, V, Zn, Zr 1. HF, Aqua regia 

2. H3BO3

Multimode/ 

LP closed

1. 8 min at 300 W, 

4 min at 600 W, 

7 min at 480 W 

2. 6 min at 600 W

ICP-OES F20
NIST 

Buffalo river sediment 

2704

As, Cr, Cu, Mn, Ni, Pb, Se, Zn HNO3, H2O Multimode/ 

LP closed

12 min to 100 psi (651 W), 

30 min at 100 psi (651 W, 181°C)

F-AAS 

ETV-AAS

F46
Ag, Al, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, Sr, V, Zn HNO3 Multimode/ 

LP closed

5.5 min to 175°C (574 W), 

4.5 min at 175-180°C (574 W)

ICP-OES F47, F48
Ag, As, Ba, Cd, Cu, Cr, Hg, Ni, Pb, Se, Tl, Zn A. 

HNO3 

B. 

HNO3, HF

Multimode/ 

LP closed 

Multimode/ 

LP closed

2:20 min at 600 W, 

9:25 min at 480 W 

Same as A

ETV-AAS 

CV-AAS

F49
Ag, As, Ba, Cd, Cu, Cr, Hg, Ni, Pb, Sb, Se, Tl, Zn Extraction: 

HNO3 

Total: 

HNO3, HF

Multimode/ 

LP closed 

Multimode/ 

LP closed

2:20 min at 604 W, 

9:25 min at 570 W 

Same as extraction

  F50
NIST 

Buffalo river sediment 

2704 

(contd.)

Ba, Cd, Cr, Co, Cu, Pb, Ni, Sr, V, Zn HNO3 Multimode/ 

LP closed

5.5 min to 175°C (574 W), 

4.5 min at 175-180°C (574 W)

F51
Co, Cr, Cu, Ni, Pb, Zn A. 

1. HF, HNO3, HCl 

2. H3BO3 

B. 

HNO3 

C. 

Same as A 

D. 

1. HF, HNO3 

2. H3BO3 

E. 

HNO3, HCl 

F. 

Same as A 

G. 

Same as E 

H. 

HF, HNO3, HCl 

I. 

HNO3, H2O2 

J. 

HNO3, H2O2, HF 

K. 

1. HNO3, H2O2, HF 

2. H3BO3 

L. 

Acetic acid 

M. 

Same as E 

N. 

Same as H

Multimode/ 

LP closed 

(all same)

8 min at 630 W, 

15 min at 315 W 

Same as A 

10 min at 630 W, 

20 min at 473 W, 

5 min at 158 W 

Same as C 

Same as C 

10 min at 630 W, 

20 min at 410 W, 

5 min at 189 W 

10 min at 630 W, 

20 min at 441 W, 

5 min at 158 W 

15 min at 630 W 

Same as G 

Same as G 

Same as G 

Same as G 

7 min at 441 W, 

5 min at 630 W 

Same as M

F-AAS 

ETV-AAS

F52, 

F53

NIST 

Buffalo river sediment 

2704 (contd.)

Al, As, Ba, Be, Ca, Co, Cr, Cu, Fe, Hg, Mg, Mn, Ni, P, Pb, S, Se, Tl, U, V, Zn HNO3 Multimode/ 

LP closed

Heat to 175°C within 5.5 min, 

4.5 min at 175-180°C

ICP-MS 

ETV-AAS

F54
Cd, Cr, Cu, Ni, Pb, Zn A. 

HNO3 

B. 

HNO3, HF

Multimode/ 

LP closed 

Multimode/ 

LP closed

Heat to 175°C within 5.5 min, 

4.5 min at 175-180°C 

Heat to 175°C within 5.5 min, 

9.5 min at 175-180°C

F-AAS 

ETV-AAS 

ICP-MS

F55
NIST 

Montana soil 

highly elevated 

traces 

2710

Cd, Cr, Cu, Ni, Pb, Zn A. 

HNO3 

B. 

HNO3, HF

Multimode/ 

LP closed 

Multimode/ 

LP closed

Heat to 175°C within 5.5 min, 4.5 min at 175-180°C 

Heat to 175°C within 5.5 min, 9.5 min at 175-180°C

F-AAS 

ETV-AAS 

ICP-MS

F55
NIST 

Montana soil 

moderately elevated 

traces 

2711

Cd, Cr, Cu, Ni, Pb, Zn A. 

HNO3 

B. 

HNO3, HF

Multimode/ 

LP closed 

Multimode/ 

LP closed

Heat to 175°C within 5.5 min, 4.5 min at 175-180°C 

Heat to 175°C within 5.5 min, 9.5 min at 175-180°C

F-AAS 

ETV-AAS 

ICP-MS

F55
NIST 

Peruvian soil 4355

As, Cr, Cu, Mn, Ni, Pb, Se, Zn HNO3, H2O Multimode/ 

LP closed

12 min to 100 psi (651 W), 

30 min at 100 psi (651 W/181°C)

F-AAS 

ETV-AAS

F46
Ag, Al, B, Ba, Be, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, Sr, V, Zn HNO3 Multimode/ 

LP closed

5.5 min to 175°C (574 W), 

4.5 min at 175-180°C (574 W)

ICP-OES F47,F48
NRCC 

Marine sediment BCSS-1

Al, Ba, Be, Ca, Cd, Cr, Cu, Fe, Hg, Mg, Mn, Na, Ni, P, Pb, Sr, Ti, V, Zn HNO3, HCl Multimode/ 

LP closed

1-20 min at 720 W ICP-OES 

ETV-AAS

F56
Al, As, Ba, Be, Ca, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, S, Sb, Ti, V, Zn A. 

Aqua regia 

B. 

1. Aqua regia, HF 

2. H3BO3

Multimode/ 

LP closed 

Multimode/ 

LP closed

2 min at 600 W, 

5 min at 360 W, 

15 min at 180 W 

1. 1 min at 600 W, 

5 min at 360 W, 

15 min at 180 W 

2. 15 min at 330 W

ICP-OES 

NAA

F23
NRCC 

Marine sediment 

MESS-1

Al, Cr, K, Li, Si, Ti, Zr 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
Si, Al, Zn, Cr HCl, HNO3, HF Multimode/ 

LP closed

50 - 60 sec at 700 W F-AAS F27
Al, Ba, Be, Ca, Cd, Cr, Cu, Fe, Hg, Mg, Mn, Na, Ni, P, Pb, Sr, Ti, V, Zn HNO3, HCl Multimode/ 

LP closed

1-20 min at 720 W ICP-OES 

ETV-AAS

F56
Al, As, Ba, Be, Ca, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, S, Sb, Ti, V, Zn A. 

Aqua regia 

B. 

1. Aqua regia, HF 

2. H3BO3

Multimode/ 

LP closed 

Multimode/ 

LP closed

2 min at 600 W, 

5 min at 360 W, 

15 min at 180 W 

1. 1 min at 600 W, 

5 min at 360 W, 

15 min at 180 W 

2. 15 min at 330 W

ICP-OES 

NAA

F23
As, Cd, Co, Cr, Cu, Mn, Ni, Pb, Sn, V, Zn HNO3, HF Multimode/ 

MP closed

25 min at 210 W ETV-AAS 

F-AAS

F57
As, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Se, Zn A. 

HNO3, HF, HClO4 

B. 

Same as A

Multimode/ 

LP closed 

Multimode/ 

LP closed

100% power to 60-65 psi, 

20 min at 65 psi 

Heat as in A, vent, repeat A

F-AAS 

ETV-AAS 

ICP-OES

F58
NRCC 

Marine Sediment 

PACS-1

As, Cr, Cu, Mn, Ni, Pb, Se, Zn HNO3, H2O Multimode/ 

LP closed

12 min to 100 psi (651 W), 

30 min at 100 psi (651 W/181°C)

F-AAS 

ETV-AAS

F46
Al, As, Ba, Be, Ca, Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Pb, S, Sb, Ti, V, Zn A. 

Aqua regia 

B. 

1. Aqua regia, HF 

2. H3BO3

Multimode/ 

LP closed 

Multimode/ 

LP closed

2 min at 600 W, 

5 min at 360 W, 

15 min at 180 W 

1. 1 min at 600 W, 

5 min at 360 W, 

15 min at 180 W 

2. 15 min at 330 W

ICP-OES 

NAA

F23
NRCC 

Soil 

SO-2

Cu, Zn HNO3, HF Multimode/ 

LP closed

15 min at 105 W, vent, 

7 min at 350 W

F-AAS F59
NRCC 

Soil 

SO-3

Cu, Zn HNO3, HF Multimode/ 

LP closed

15 min at 105 W, vent, 

7 min at 350 W

F-AAS F59
NRCC 

Soil 

SO-4

Cu, Zn HNO3, HF Multimode/ 

LP closed

15 min at 105 W, vent, 

7 min at 350 W

F-AAS F59
RMA 

Standard 

soil

Ag, As, Ba, Cd, Cu, Cr, Hg, Ni, Pb, Se, Tl, Zn A. 

HNO3 

B. 

HNO3, HF

Multimode/ 

LP closed 

Multimode/ 

LP closed

2:20 min at 600 W, 

9:25 min at 480 W 

Same as A

ETV-AAS 

CV-AAS

F49
Ag, As, Ba, Cd, Cu, Cr, Hg, Ni, Pb, Sb, Se, Tl, Zn Extraction: 

HNO3 

Total: 

HNO3, HF

Multimode/ 

LP closed 

Multimode/ 

LP closed

2:20 min at 604 W, 

9:25 min at 570 W 

Same as extraction

F50
USGS 

Soil GXR-2

Al, K, Li, Si, Ti, Zr 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
Nb, Mo, Ta, W 1. HNO3 

2. HF, H2O

Multimode/ 

MP closed

1. 30 min at 200 W 

2. 1 min at 1000 W, 

30 min at 450 W

ID-ICP-MS F9
USGS 

Fe-Mn-W rich hot spring deposit 

GXR-3

Al, K, Li, Mg, Si, Ti 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
USGS 

Soil GXR-5

Al, Cr, K, Li, Si, Ti, Zr 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
Nb, Mo, Ta, W 1. HNO3 

2. HF, H2O

Multimode/ 

MP closed

1. 30 min at 200 W 

2. 1 min at 1000 W, 

30 min at 450 W

ID-ICP-MS F9
USGS 

Marine 

sediment MAG-1

Nb, Mo, Ta, W 1. HNO3 

2. HF, H2O

Multimode/ 

MP closed

1. 30 min at 200 W 

2. 1 min at 1000 W, 

30 min at 450 W

ID-ICP-MS F9
Al, K, Li, Si, Ti, Y, Zr 1. HNO3, HCl, HF 

2. H3BO3

Multimode/ 

LP closed

1. 2.5 min at 650 W 

2. 10 min at 650 W

ICP-OES F2
Back to Appendices
Back to Text