Appendix 1. Biological Reference Materials

Appendix 1.2 Botanical
Matrix Analytes Reagents Cavity/Vessel Conditions Detection Ref.

BCR

Aquatic plant

60

Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn HNO3, HCl

Multimode/

LP closed

15 min at 300 W,

5 min at 0 W,

20 min at 510 W

F-AAS

ETV-AAS

F-OES

E72
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

ETV-AAS E9
Fe

A.

HNO3

B.

1. HNO3

2. HF


Multimode/

HP closed

Multimode/

HP closed


30 sec at 700 W

Same as A

ETV-AAS E6
Cd, Cu, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

ETV-AAS E5

BCR

Aquatic moss 61

(contd.)

Cd HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

ETV-AAS E4
Cu, Cd, Mn, Pb, Zn HNO3

Multimode/

HP closed

30 sec at 665 W

F-OES

F-AAS

ICP-OES

E49
Ni HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

ICP-OES E10, E11

BCR

Aquatic moss

61

Cu, Cd, Mn, Pb, Zn HNO3

Multimode/

HP closed

30 sec at 665 W

F-OES

F-AAS

ICP-OES

E49
Fe

A.

HNO3

B.

1. HNO3

2. HF


Multimode/

HP closed

Multimode/

HP closed


30 sec at 700 W

Same as A

ETV-AAS E6

BCR

Aquatic moss 61

(contd.)

Cd HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

ETV-AAS E4
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

ETV-AAS E9
Ni HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

ICP-OES E10, E11

BCR

Olive blossom

62

Cu, Cd, Mn, Pb, Zn HNO3

Multimode/

HP closed

30 sec at 665 W

F-OES

F-AAS

ICP-OES

E49
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

ETV-AAS E9
Ni HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

ICP-OES E10, E11
Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn HNO3, HCl

Multimode/

LP closed

15 min at 300 W,

5 min at 0 W,

20 min at 510 W

F-AAS

ETV-AAS

F-OES

E72
Cd, Cu, Mn, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

ETV-AAS E5
Pb HNO3, HCl

Multimode/

flow through

18 sec at 700 W

(0.4 mL coil, 1.5 mL min-1)

ETV-AAS E8
Cu, Mg, Pb HNO3

Multimode/

LP closed

3 min at 650 W ETV-AAS E3

BCR

Spruce needles

101

Cd, Cu, Pb HNO3, HClO4, H2SO4

Multimode/

HP closed.

10 min at 85 bar DP-ASV E1
Al, Ca, Mg, Mn, P, Zn

1. HNO3

2. HNO3

3. H2O2

4. H2O

Single mode/

open

1. 5 min at 10 W,

10 min at 30 W,

10 min at 60 W

2. 10 min 60 W

3. 5 min at 60 W

4. 5 min at 50 W

ICP-OES

ICP-MS

HPLC-ICP-MS

E13

BCR

Wholemeal flour

189

Cd, Cu, Fe, Mn, Zn

A.

HNO3, H2O2

B.

HNO3, H2O2



C.

HNO3, HClO4


Multimode/

HP closed

Multimode/

HP closed



Multimode/

HP closed


75 sec at 665 W

1 min at 250 W,

2 min at 0 W,

2 min at 250 W,

2 min at 400 W,

2 min at 600 W

150 sec at 950 W,

60 sec at 0 W,

90 sec at 300 W,

90 sec at 500 W,

90 sec at 700 W,

90 sec at 850 W

ETV-AAS E6

BCR

Ulva lactuca

279

Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn HNO3

Multimode/

LP closed

5 min at 300 W,

5 min at 0 W,

5 min at 300 W,

5 min at 450 W,

5 min at 0 W,

5 min at 450 W

F-AAS

ETV-AAS

F-OES

E72

BCR

White clover

402

Co, Mo

A.

1. HNO3

2. HNO3

3. H2O2

4. H2O

B.

1. HNO3, HCl

2. HNO3, HCl

3. H2O2

4. H2O


Single mode/

open




Single mode/

open


1. 5 min at 10 W,

10 min at 30 W,

10 min at 60 W

2. 10 min 60 W

3. 5 min at 60 W

4. 5 min at 50 W

1. 5 min at 40 W,

10 min at 50 W

2. 20 min 54 W

3. 5 min at 40 W

4. 5 min at 50 W

ICP-OES

ICP-MS

HPLC-ICP-MS

E13

IAEA

Hay powder

V10

Mo

1. HNO3

2. Perhydrol

Multimode/

open then Multimode/

LP closed

1. 30 min at 180W (open),

10 min at 420W,

10 min at 600W

2. 10 min at 600W

MS E73
I HNO3, N2H4

Multimode/

HP closed

35 sec at 675 W NAA E17

IAEA

Hay powder

V10

(contd.)

Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn HNO3, HCl

Multimode/

LP closed

15 min at 300 W,

5 min at 0 W,

20 min at 510 W

F-AAS

ETV-AAS

F-OES

E72
Hg

1. HNO3

2. H2O2

Multimode/

HP closed

3 min at 450 W Radiochemical E30
Ag, Ba, Bi, Cd, Co, Cr, Cs, Cu, Ga, Hg, Li, Mo, Ni, Pb, Rb, Sn, Sr, Th, U, Zn, Zr HNO3

Multimode/

HP closed

1 min at 300 W (x3-5),

7 min at 300 W, 2 min at 600 W

ICP/MS E74

MOE

Vegetation

V85-1

Al, Ba, Ca, Cu, Fe, Mg, Mn, Zn Aqua regia

Multimode/

stopped flow

2 min at 720 W or

32 min at 720 W

ICP-OES E58

MOE

Norway maple

Al, Ba, Ca, Cu, Fe, Mg, Mn, Pb, Zn

A.

Aqua regia

B.

Aqua regia


Multimode/

stopped flow

Multimode/

open


2 min at 720 W

2 min at 720 W,

2 min at 0 W,

2 min at 720 W,

2 min at 0 W,

2 min at 720 W

(with water load)

ICP-OES E58

MOE

White birch

Al, Ba, Ca, Cd, Cu, Fe, Mg, Mn, Zn

A.

Aqua regia

B.

Aqua regia


Multimode/

stopped flow

Multimode/

open


2 min at 720 W

2 min at 720 W,

2 min at 0 W,

2 min at 720 W,

2 min at 0 W,

2 min at 720 W

(with water load)

ICP-OES E58

NIES

Pepperbush 1

Ca, Fe, Mg, Zn HNO3

Multimode/

flow through

100 sec at 525 W

(10 mL coil, 6 mL min-1)

F-AAS E39
Ca, Cu, Fe, Mg, Mn, Zn HNO3, HClO4, HCl, HF

Multimode/

MP closed

14 min intermittent 200 W F-AAS E27

NIES

Pepperbush

1

(contd.)

Co HNO3, HCl

Multimode/

MP closed

5 min at 600 W

(50 mL water load),

5 min at 600 W

(no load)

UV-Vis E75
Cd, Co, Cu, Ni, Pb

HNO3, HCl,

HClO4, HF

Multimode/

HP closed

5 min at 200 W (water load),

3.5 min at 200 W (no load)

ICP-OES

ETV-ICP

E28
Cd, Cu, Fe, Mn, Zn

A.

HNO3, H2O2

B.

HNO3, H2O2



C.

HNO3, HClO4


Multimode/

HP closed

Multimode/

HP closed



Multimode/

HP closed


75 sec at 665 W

1 min at 250 W,

2 min at 0 W,

2 min at 250 W,

2 min at 400 W,

2 min at 600 W

150 sec at 950 W,

60 sec at 0 W,

90 sec at 300 W,

90 sec at 500 W,

90 sec at 700 W,

90 sec at 850 W

ETV-AAS E6
Fe, Mn, Zn HNO3

Multimode/

flow through

-

F-AAS

ICP-OES

ICP/MS

E66
Cd

1. HNO3, HClO4, HCl, HF

2. HClO4

Multimode/

MP closed

1. 5 min at 200 W

(using water load),

4 min at 200 W (no load)

F-AAS E76

NIES

Tea leaves

7

Ca, Cu, Fe, Mg, Mn, Zn HNO3, HClO4, HCl, HF

Multimode/

MP closed

14 min intermittent 200 W F-AAS E27
Co HNO3, HCl

Multimode/

LP closed

5 min at 600 W

(50 mL water load),

5 min at 600 W (no load)

UV-Vis E75
Cd, Co, Cu, Ni, Pb

HNO3, HCl,

HClO4, HF

Multimode/

MP closed

5 min at 200 W (water load),

3.5 min at 200 W (no load)

ICP-OES

ETV-ICP

E28

NIES

Sargasso

9

Ca, Fe, Mg, Zn HNO3

Multimode/

flow through

100 sec at 525 W

(10 mL coil, 6 mL min-1)

F-AAS E39
Fe, Mn, Zn HNO3

Multimode/

flow through

-

F-AAS

ICP-OES

ICP/MS

E66
As, Cr, V HNO3, H2O2

Multimode/

LP closed

3 min at 300 W ICP/MS E77
Ca, Cu, Fe, Mg, Mn, Zn HNO3, HClO4, HCl, HF

Multimode/

MP closed

14 min intermittent 200 W F-AAS E27

NIES

Rice flour

10

Mo

1. HNO3

2. Perhydrol

Multimode/

open then Multimode/

LP closed

1. 30 min at 180W (open),

10 min at 420W,

10 min at 600W

2. 10 min at 600W

MS E73
Cd

1. HNO3, HClO4, HCl, HF

2. HClO4

Multimode/

MP closed

1. 5 min at 200 W (using load), 4 min at 200 W (no load) F-AAS E76

NIST

Apple leaves

1515

Ba, Ce, Co, Cr, Eu, Fe, Hf, K, La, Na, Sb, Sc, Sm, Sr, Tb, Zn

1. HNO3, HF

2. HClO4

Multimode/

LP closed

1. 20 min at 373 W (9 vessels)

2. 20 min at 545 W (6 vessels)

NAA E78

NIST

Peach leaves

1547

Ba, Ce, Co, Cr, Eu, Fe, Hf, K, La, Na, Sb, Sc, Sm, Sr, Tb, Zn

1. HNO3, HF

2. HClO4

Multimode/

LP closed

1. 20 min at 373 W (9 vessels)

2. 20 min at 545 W (6 vessels)

NAA E78

NIST

Wheat flour

1567

Mo

1. HNO3

2. Perhydrol

Multimode/

open then Multimode/

LP closed

1. 30 min at 180 W (open),

10 min at 420 W,

10 min at 600 W

2. 10 min at 600W

MS E73
Se

1. HNO3

2. H2SO4, HClO4

3. H2O

Single mode/

open

1. 15 min at 45 W,

10 min at 75 W

2. 10 min at 90 W,

35 min at 120 W

3. 8 min at 120 W

ETV-AAS E18
Cd HNO3

Multimode/

LP closed

2 min on defrost three times ETV-AAS E40
Hg HNO3

Multimode/

LP closed

8 min at 140 W,

8 min at 280 W,

4 min at 420 W

CV-AAS E2
Ba, Ca, K, Mg, Mn, Na, P, S, Zn

1. HNO3

2. H2O2

Multimode/

open

1. 30 min at 540 W

2. 30 min at 540 W

ICP-OES E42
Residual amino acids HNO3

Multimode/

LP closed

Varied Fluorimetric E43

NIST

Wheat flour

1567

(contd.)

Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
Se HNO3

Multimode/

LP closed

(1.5 h at 60°C on hotplate)

4 min at 287 W

ETV-AAS E44
Ca, Cu, Fe, K, Mn, Zn HNO3

Multimode/

HP closed

30 sec at 665 W

F-OES

F-AAS

ICP-OES

E49
As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn HNO3, H2O2

Multimode/

MP closed

(Max 350 psi - power cutoff at 150 psi)

4 min at 296 W,

8 min at 360 W

F-AAS

ICP/MS

ICP-OES

E79
Cu, Fe, K, Mn, Zn HNO3

Multimode/

LP closed

8 min at 150 W

F-AAS

ETV-AAS

SIMAAC

E54
Ca, Cu, Fe, K, Mg, Mn, P, Zn HNO3, HCl

Multimode/

LP closed

5 min at 300 W,

5 min at 0 W,

5 min at 300 W,

5 min at 450 W,

5 min at 0 W,

5 min at 450 W

ICP-OES E80

NIST

Wheat flour

1567

(contd.)

Cd, Cu, Fe, Mn

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

ETV-AAS E5
Ca, Fe, K, Mg, Mn, Na, P, S

1. HNO3

2. H2O2

3. HCl

Multimode/

LP closed

1. 30 min at 540 W,

5 min at 0 W

2. 5 min at 0 W,

15 min at 540 W,

5 min at 0 W

3. 10 min at 180 W

ICP-OES E81

NIST

Rice flour 1568

Ba, Ca, K, Mg, Mn, Na, P, S, Zn

1. HNO3

2. H2O2

Multimode/

open

1. 30 min at 540 W

2. 30 min at 540 W

ICP-OES E42
Residual amino acids HNO3

Multimode/

LP closed

Varied Fluorimetry E43
Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
B HNO3, H2O2

Multimode/

HP closed

2 min at 100W,

2 min at 0 W,

5 min at 250 W,

3 min at 0 W,

5 min at 500 W,

15 min at 250 W

UV-Vis

Fluorimetry

ICP-OES

ICP/MS

E64
As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn HNO3, H2O2

Multimode/

MP closed

(Max 350 psi - power cutoff at 150 psi)

4 min at 296 W,

8 min at 360 W

F-AAS

ICP/MS

ICP-OES

E79
Cr, Hg, Se

1. HNO3

2. H2O2

Multimode/

HP closed

1. 3 min at 450 W Radiochemical E30

NIST

Spinach 1570

I HNO3, N2H4

Multimode/

HP closed

35 sec at 675 W NAA E17
Ba, Ca, K, Mg, Mn, Na, P, S, Zn

1. HNO3

2. H2O2

Multimode/

open

1. 30 min at 540 W

2. 30 min at 540 W

ICP-OES E42

NIST

Spinach

1570

(contd.)

Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
Al, Ba, Ca, Cr, Cu, Fe, K, Li, Mg, Mn, Na, P, Si, Sr, Ti, Zn

1. Aqua regia

2. HBO3

Multimode/

MP closed

(evacuated slightly at start)

1. 3 min at 625 W ICP-OES E55

NIST

Orchard leaves

1571

I HNO3, N2H4

Multimode/

HP closed

35 sec at 675 W NAA E17
Cd HNO3

Multimode/

LP closed

2 min on defrost three times ETV-AAS E40
As HNO3, HClO4, H2SO4

Multimode/

HP closed.

10 min at 85 bar DP-ASV E1
Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
As, Co, Cr, Cu, Ni, Pb, Se, Zn HNO3, HClO4

Multimode/

open

600 W until first signs of HClO4 fumes

F-AAS

NAA

E46, E47, E48

NIST

Orchard leaves

1571 (contd.)

Cd

1. HNO3, HClO4, HCl, HF

2. HClO4

Multimode/

MP closed

1. 5 min at 200 W

(using water load),

4 min at 200 W (no load)

F-AAS E76
Al, Ba, Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, P , S, Zn HNO3

Multimode/

LP closed

10 min at 70 psi,

15 min at 450 W

NAA

ICP-OES

E22
Po HNO3

Multimode/

MP closed

60 min at 60 W, cool

60 min at 60 W

a-spectrometry E82
Cd, Co, Fe, Ni, Pb HNO3, HF

Multimode/

LP closed

1 min at 240 W, cool (x15)

ETV-AAS

X-ray Fluorescence

E83
Al, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, P, Zn

1. Aqua regia

2. HBO3

Multimode/

MP closed

(evacuated slightly at start)

3 min at 625 W ICP-OES E55
Se HNO3, HClO4 Not reported - Fluorimetry E65
Cu, Pb, Zn HNO3, HClO4

Multimode/

open

3 min at 600 W ETV-AAS E84
Al, Ba, Ca, Cu, Fe, Mg, Pb, Zn

A.

Aqua regia

B.

Aqua regia


Multimode/

stopped flow

Multimode/

open


2 min at 720 W

2 min at 720 W,

2 min at 0 W,

2 min at 720 W,

2 min at 0 W,

2 min at 720 W

(with water load)

ICP-OES E58
Al HNO3

Multimode/

HP closed

1 min at 150 W, 30 min at 0 W,

1 min at 450 W

ICP-OES E20

NIST

Citrus leaves

1572

Al, Ag, As, Ba, Ca, Cl, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, N, Na, Ni, P, Pb, Rb, S, Sb, Si, Sr, Ti, Zn HNO3, HClO4

Multimode/

MP closed

15 min at 0 W,

10 min at 150 W,

10 min at 450 W,

15 min at 0 W

ICP-OES E31
I HNO3, N2H4

Multimode/

HP closed

35 sec at 675 W NAA E17
Cd HNO3

Multimode/

LP closed

2 min on defrost three times ETV-AAS E40
Ba, Ca, K, Mg, Mn, Na, P, S, Zn

1. HNO3

2. H2O2

Multimode/

open

1. 30 min at 540 W

2. 30 min at 540 W

ICP-OES E42

NIST

Citrus leaves

1572

(contd.)

Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
- HNO3

Multimode/

LP closed

10 min at 360 W, 5 min at 480 W - E85
Cd

1. HNO3, HClO4, HCl, HF

2. HClO4

Multimode/

MP closed

1. 5 min at 200 W

(using water load),

4 min at 200 W (no load)

F-AAS E76
Hg HNO3

Multimode/

LP closed

800 W until 75°C,

3 min at 75°C

CV-FANES E86
Hg HNO3

Multimode/

HP closed

90 sec at 600 W

(estimated power)

CV-AAS E87
Al HNO3

Multimode/

MP closed

4 min at 200 W, cool,

4 min at 350 W, cool,

8 min at 250 W, cool,

10 min at 400 W (x4)

ETV-AAS E88
Sr HNO3

Multimode/

open

Heat until dryness. ID (stable isotope dilution activation analysis) E89
As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn HNO3, H2O2

Multimode/

MP closed

(Max 350 psi - power cutoff at 150 psi)

4 min at 296 W,

8 min at 360 W

F-AAS

ICP/MS

ICP-OES

E79

NIST

Citrus leaves

1572

(contd.)

Ca, Cu, Fe, K, Mg, Mn, P, Zn HNO3, HCl

Multimode/

LP closed

5 min at 300 W,

5 min at 0 W,

5 min at 300 W,

5 min at 450 W,

5 min at 0 W,

5 min at 450 W

ICP-OES E80
As, Cr, Hg, Sb

1. HNO3

2. H2O2

Multimode/

HP closed

3 min at 450 W Radiochemical E30
Ba, Ca, Cu, Mg, Mn, Zn

1. HNO3, HF

2. HBO3

Multimode/

LP closed

2 min at 240 W,

2 min 360 W,

16 min at 160°C (600W)

ICP-OES E90
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

ETV-AAS E9
Ni HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

ICP-OES E10, E11
Al, Ca, Fe, K, Mg, Mn, Na, P, S

1. HNO3

2. H2O2

3. HCl

Multimode/

LP closed

1. 30 min at 540 W,

5 min at 0 W

2. 5 min at 0 W,

15 min at 540 W,

5 min at 0 W

3. 10 min at 180 W

ICP-OES E81
P

1. HNO3, HClO4

2. HClO4

Multimode/

open (with scrubber)

1. 15 min at 750 W ICP-OES E91
Al HNO3, H2SO4

Multimode/

MP closed

5 min at 1170 W IC E92
As HNO3, H2O2

Multimode/

HP closed

- ICP/MS E93
Ni HNO3, HCl

Multimode/

HP closed

4 min at 460 W,

10 min at 180 W

(10 mL water load)

ICP-OES E94

NIST

Citrus leaves

1572

(contd.)

Cu, Fe, Mn, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

ETV-AAS E5
Ca, Fe, K, Mg, Mn, Zn -

Multimode/

open

30 min at 650 W F-AAS E95
Ca, Cu, Fe, Mg, Mn, K, Na, Zn

1. HNO3

2. HNO3

3. H2O2

Single mode/

open

1. 10 min at 60 W,

5 min at 80 W

2. 5 min at 70 W

3. 3 min at 60 W

F-AAS E67
Al HNO3

Multimode/

HP closed

1 min at 150 W, 30 min at 0 W,

1 min at 450 W

ICP-OES E20
Al, As, Ba, Cr, Cu, Fe, K, Mg, Mn, Ni, P, Pb, Rb, Sr, Zn

1. HNO3

2. HF

3. H2O2

Multimode/

HP closed

1. 5 min at 300 W,

2 min at 600 W,

2 min at 0 W,

3 min at 300 W

2. 5 min at 300 W,

2 min at 600 W,

2 min at 0 W

3. 5 min at 300 W,

2 min at 600 W,

2 min at 0 W,

3 min at 300 W

DCP-OES

F-AAS

E96

NIST

Tomato leaves 1573

Cu, Mn HNO3, H2O2

Multimode/

flow through

2 min at 650 W (digestate recirculated through oven) F-AAS E97
Ba, Ca, K, Mg, Mn, Na, P, S, Zn

1. HNO3

2. H2O2

Multimode/

open

1. 30 min at 540 W

2. 30 min at 540 W

ICP-OES E42
Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
As, Cr, Cu, Fe, Mn, Pb, Rb, Sr, Th, U, Zn

1. HNO3

2. HF

Multimode/

MP closed

1. Leave overnight,

3 min at 130 W,

6 min at 195 W,

6 min at 260 W,

6 min at 325 W, cool, vent

2. 6 min at 325 W

ICP/MS E98
Cd

1. HNO3, HClO4, HCl, HF

2. HClO4

Multimode/

MP closed

1. 5 min at 200 W

(using water load),

4 min at 200 W (no load)

F-AAS E76

NIST

Tomato leaves

1573

(contd.)

Ca, Cu, Fe, K, Mn, P, Pb, Zn HNO3

Multimode/

HP closed

30 sec at 665 W

F-OES

F-AAS

ICP-OES

E49
Sr HNO3

Multimode/

open

Heat until dryness. ID (stable isotope dilution activation analysis) E89
Al, Ba, Ca, Cd, Cr, Fe, K, Mg, Mn, Na, P

1. Aqua regia

2. HBO3

Multimode/

MP closed

(evacuated slightly at start)

3 min at 625 W ICP-OES E55
Cd, Co, Fe, Ni, Pb HNO3, HF

Multimode/

LP closed

1 min at 240 W, cool

(x15)

ETV-AAS

X-ray Fluorescence

E83
Cr, Hg, Se

1. HNO3

2. H2O2

Multimode/

HP closed

3 min at 450 W Radiochemical E30
Fe

A.

HNO3

B.

1. HNO3

2. HF


Multimode/

HP closed

Multimode/

HP closed


30 sec at 700 W

Same as A

ETV-AAS E6
Ca, Fe, K, Mg, Mn, Zn -

Multimode/

open

30 min at 650 W F-AAS E95
B HNO3, H2O2

Multimode/

HP closed

2 min at 100W,

2 min at 0 W,

5 min at 250 W,

3 min at 0 W,

5 min at 500 W,

15 min at 250 W

UV-Vis

Fluorimetry

ICP-OES

ICP/MS

E64

NIST

Tomato leaves

1573

(contd.)

Mn HNO3

Multimode/

flow through

2 sec at 650 W

(0.5 mL coil, 15.4 mL min-1)

F-AAS E99
Al, As, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sb, Se, V, Zn Aqua regia

Multimode/

LP closed

10 min at 300 W, 5 min at 600 W, 10 min at 480 W

ICP-OES

ETV-AAS

E56
Ca, Fe, K, Mg, Mn, Na, P, S

1. HNO3

2. H2O2

3. HCl

Multimode/

LP closed

1. 30 min at 540 W,

5 min at 0 W

2. 5 min at 0 W,

15 min at 540 W,

5 min at 0 W

3. 10 min at 180 W

ICP-OES E81

NIST

Pine needles 1575

Pb HNO3, HCl

Multimode/

flow through

18 sec at 700 W

(0.4 mL coil, 1.5 mL min-1)

ETV-AAS E8
Ba, Ca, K, Mg, Mn, Na, P, S, Zn

1. HNO3

2. H2O2

Multimode/

open

1. 30 min at 540 W

2. 30 min at 540 W

ICP-OES E42

NIST

Pine needles

1575

(contd.)

Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
Al, As, Cr, Cu, Fe, Hg, Mn, Pb, Rb, Sr, Th, U

1. HNO3

2. HF

Multimode/

MP closed

1. Leave overnight,

3 min at 130 W,

6 min at 195 W,

6 min at 260 W,

6 min at 325 W, cool, vent

2. 6 min at 325 W

ICP/MS E98
Ca, Cu, Fe, K, Mn, P, Pb HNO3

Multimode/

HP closed

30 sec at 665 W

F-OES

F-AAS

ICP-OES

E49
Hg HNO3

Multimode/

LP closed

800 W until 75°C,

3 min at 75°C

CV-FANES E86

NIST

Pine needles 1575

(contd.)

As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn HNO3, H2O2

Multimode/

MP closed

(Max 350 psi - power cutoff at 150 psi)

4 min at 296 W,

8 min at 360 W

F-AAS

ICP/MS

ICP-OES

E79
Al, Ba, Ca, Cr, Fe, K, Mg, Mn, Na, P, Zn

1. Aqua regia

2. HBO3

Multimode/

MP closed

(evacuated slightly at start)

1. 3 min at 625 W ICP-OES E55
Al, As, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sb, Se, V, Zn Aqua regia

Multimode/

LP closed

10 min at 300 W, 5 min at 600 W, 10 min at 480 W

ICP-OES

ETV-AAS

E56
Cd, Co, Fe, Ni, Pb HNO3, HF

Multimode/

LP closed

1 min at 240 W, cool

(x15)

ETV-AAS

XRF

E83

NIST

Pine needles

1575

(contd.)

Ca, Fe, K, Mg, Mn, Na, P, S

1. HNO3

2. H2O2

3. HCl

Multimode/

LP closed

1. 30 min at 540 W,

5 min at 0 W

2. 5 min at 0 W,

15 min at 540 W,

5 min at 0 W

3. 10 min at 180 W

ICP-OES E81
Ca, K, Mg, P

1. HNO3, HClO4

2. HClO4

Multimode/

open (with scrubber)

15 min at 750 W ICP-OES E91
Pb HNO3

Multimode/

LP closed

3 min at 650 W ETV-AAS E3
Al HNO3

Multimode/

LP closed

40 sec at 700 W,

10 min at 0 W (vent),

60 sec at 700 W,

10 min at 0 W (vent),

90 sec at 700 W,

5 min at 70 W

ETV-AAS E63
Cu

1. HNO3

2. H2O2

Multimode/

LP closed

9.5 min at 540 W F-AAS E100

NIST

Pine needles

1575

(contd.)

Cu, Fe, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

ETV-AAS E5
B HNO3, H2O2

Multimode/

HP closed

2 min at 100W,

2 min at 0 W,

5 min at 250 W,

3 min at 0 W,

5 min at 500 W,

15 min at 250 W

UV-Vis

Fluorimetry

ICP-OES

ICP/MS

E64
Ca, Fe HNO3, HCl, HF

Multimode/

MP closed

15 min at 540 W DCP-OES E101
Al, As, Ba, Cr, Cu, Fe, K, Mg, Mn, Ni, P, Pb, Rb, Sr, Zn

1. HNO3

2. HF

3. H2O2

Multimode/

HP closed

1. 5 min at 300 W,

2 min at 600 W,

2 min at 0 W,

3 min at 300 W

2. 5 min at 300 W,

2 min at 600 W,

2 min at 0 W

3. 5 min at 300 W,

2 min at 600 W,

2 min at 0 W,

3 min at 300 W

DCP-OES

F-AAS

E96

NIST

Corn stalks

8412

Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux off

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35

NIST

Corn kernel

8413

Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

FI-ICP-OES E35
Pb HNO3, V2O5

Multimode/

HP closed

90 sec at 460 W ETV-AAS E104
Hg HNO3

Multimode/

HP closed

90 sec at 600 W CV-AAS E105

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